AlphaSeq is a multiplexed, quantitative, and versatile technology for characterizing entire protein-protein interaction networks.
TECHNOLOGY
AlphaSeq is a quantitative and versatile yeast-based platform for measuring millions of protein interactions at a library-on-library scale
Build
Mix
Bind
Fuse
Measure
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Two yeast surface display libraries are built and mixed in liquid culture
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Cells with complementary proteins bind to one another and fuseCl.ls
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Next generation sequencing is used to measure interaction strengths
AlphaSeq combines the throughput of a genetically encoded assay with the accuracy of a biophysical method by measuring protein interactions extracellularly, where the environment can be tightly controlled.
Multiplexed
Characterize whole protein interaction networks:
1 Million+ interactions
Quantitative
Measure interaction strengths with physiological relevance:
affinities from pM to µM
Versatile
