AlphaSeq is a multiplexed, quantitative, and versatile technology for characterizing entire protein-protein interaction networks.
AlphaSeq is a quantitative and versatile yeast-based platform for measuring millions of protein interactions at a library-on-library scale
Two yeast surface display libraries are built and mixed in liquid culture
Cells with complementary proteins bind to one another and fuseCl.ls
Next generation sequencing is used to measure interaction strengths
AlphaSeq combines the throughput of a genetically encoded assay with the accuracy of a biophysical method by measuring protein interactions extracellularly, where the environment can be tightly controlled.