AlphaSeq is a multiplexed, quantitative, and versatile technology for characterizing entire protein-protein interaction networks.

TECHNOLOGY

AlphaSeq is a quantitative and versatile yeast-based platform for measuring millions of protein interactions at a library-on-library scale

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Build

Mix

Bind

Fuse

Measure

  • Two yeast surface display libraries are built and mixed in liquid culture

  • Cells with complementary proteins bind to one another and fuseCl.ls 

  • Next generation sequencing is used to measure interaction strengths

As described in Younger, et al. "High-Throughput Characterization of Protein-Protein Interactions..." PNAS (2017)

AlphaSeq combines the throughput of a genetically encoded assay with the accuracy of a biophysical method by measuring protein interactions extracellularly, where the environment can be tightly controlled.

Multiplexed

Characterize whole protein interaction networks:

1 Million+ interactions

Quantitative

Measure interaction strengths with physiological relevance:

affinities from pM to µM

Versatile

Screen drugs that modulate protein interaction networks

Explore applications of the AlphaSeq platform

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